Unless otherwise specified, gene expression analysis was performed on six-week-old hiPSC neurons without astrocyte coculture. Cells were lysed in RNA BEE (Tel-test, Inc). RNA was chloroform extracted, pelleted with isopropanol, washed with 70% ethanol and resuspended in water. RNA was treated with RQ1 RNAse-free DNAse (Promega) for 30 minutes at 37°C and then the reaction was inactivated by incubation with EGTA Stop buffer at 65°C for 10 minutes.
