Chunk #14 — METHODS — Immunohistochemistry

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Repeated homotypic stress elevates 2-arachidonoylglycerol levels and enhances short-term endocannabinoid signaling at inhibitory synapses in basolateral amygdala.

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Brightfield photomicrographs were obtained using an upright Olympus SZX12 microscope coupled to an Olympus CMAD3 camera using Q-capture software. Confocal images of immunofluorescently stained sections were obtained using a Zeiss LSM510 confocal microscope. Small brightness and contrast adjustments, and figure arrangement were done using Adobe Photoshop CS.