tubes prefilled with RNAseOUT (Invitrogen), ERCC spike-in RNAs in 1:400 K dilution, sample specific RT primers for a total of 1 μL volume. Process was repeated to collect 32–64 cells in one manual cell sorting session. Cells were flash frozen in liquid nitrogen and stored at −80 °C until processed. Patch pipette was single use only and fresh pipettes were used for every single cell collected. Manual sorting resulted in negligible contaminants as shown by glial and excitatory neuronal transcripts in Fig-S1I.
