In order to determine if the GO assessment showing a decrease in the expression of genes associated with the mitotic cell cycle was accompanied by a decrease in cell growth, we assessed the proliferation rate of NPCs. Cell growth was assayed in 8 NPC lines (4 controls and 4 with 22q11.2 del). The fold change data were pooled, and control vs 22q11.2 del growth rates were analyzed as described in the methods section. As seen in Fig. 2b, there was an overall decrease in proliferation for the 22q11.2 samples at each time point. However, because of line to line variability, statistically significant differences were only seen on days 1–3 (two-tailed Student’s t-test, p = 0.01 on days 1,2; 0.03 on day 3; 0.11, 0.14 0.14 and 0.18 on days 4–7, respectively). Nevertheless, the proliferation data support the GO enrichment findings from our RNA-seq data.
