dlpf03537 homozygotes, only heterozygotes were generated in sufficient numbers for the sedation assays. The dlpf03537 heterozygotes displayed increased sedation sensitivity (Figure 2D). dallyMB950 heterozygotes and homozygotes were both more sensitive to the sedating effect of ethanol, with the homozygotes exhibiting greater sensitivity (Figure 2G and data not shown). The two mutants differed in their development of rapid tolerance [rapid tolerance was measured as the difference in sedation sensitivity between an initial exposure to ethanol, e1, and a second exposure, e2, delivered four hours later (Figure 2C)]. While dlpf03537 heterozygotes developed greater tolerance, dallyMB950 heterozygotes and homozygotes were indistinguishable from control flies (Figure 2, D and G). Ethanol absorption was unaltered in the mutants (Figure 2 legend), so we can conclude that the behavioral alterations were due to different responses to the same ethanol dose.
