by 30 to 43% in cells transfected with each of the shLmo3 plasmids compared with the shScr control (Fig. 2B). Transfection of shLmo3 plasmids had no detectable effect on the expression of the closely related Lmo family members Lmo4 (Fig. 2C), Lmo1 (Fig. 2D), and Lmo2 (data not shown). These results indicate that these shRNAs specifically attenuated expression of Lmo3 without affecting expression of the other Lmo genes.
