Collectively, these results show that the sOPTiKD system could be readily repurposed to support inducible gRNA expression and allow tightly controlled activity of CRISPR/Cas9 over a broad range of gRNA potency (Fig. S8L). To the best of our knowledge, this is the first conditional CRISPR/Cas9 approach based on inducible gRNA expression. We named this method single-step optimized inducible gene knockout, or sOPTiKO.
