0.2), Myelin basic protein (MBP) (2.6% ± 0.42), Oligodendrocyte lineage transcription factor 2 (OLIG2) (36.2% ± 1.8) and KI67 (92.4% ± 1.3)) by immunofluorescence (Figure 2C). KI67 is not a cell type-specific marker, but rather reflects the fraction of cycling cells within differentiating NSC. Indicating that the cells at an early stage of differentiation are actively dividing, expression of KI67 (98.8% ± 0.8) was also detected in D2 cells (Additional file 2). Our detection of KI67 in the majority of cells at D8 indicates that although most differentiating NSC are actively dividing, fewer than 10% of cells are post-mitotic and may include neurons (TUB III+) or non-proliferating cells committed towards neuronal cell fate. Taken together, this in vitro NSC differentiation system provided a suitable model system consisting of the three main neural cell types (neurons, astrocytes and oligodendrocytes) in the brain to study Mecp2/MeCP2 isoforms during neural differentiation.
