Subcutaneous and visceral adipose tissue volumes (SAT and VAT) were assessed (Aquarius 3D Workstation, TeraRecon Inc., San Mateo, CA). In order to identify pixels containing fat, an image display window width of −195 to −45 Hounsfield Units (HU) and a window center of −120 HU were used. The abdominal muscular wall separating the visceral from the subcutaneous compartment was manually traced. Inter-reader reproducibility was assessed by two independent readers measuring VAT and SAT on a subset of 100 randomly selected participants [73]. Inter-class correlations for inter-reader comparisons were 0.992 for VAT and 0.997 for SAT. Similar high correlations were noted for intra-reader comparisons.
