Purkinje cell dendrite self-crossings detected in single confocal planes were counted in a 7,225 μm2 region of interest assigned to middle of arbor. Purkinje arbor areas were measured using the convex-hull selection in Fiji. Calbindin-labeled Purkinje somata residing along a 635 μm segment in lobules III-VI in single confocal planes were counted to measure PC density.
