We exchanged 30 SNPs for replication testing with the SpiroMeta consortium (accompanying manuscript). No additional genotyping was required, as these SNPs were available from the SpiroMeta GWAS. We aimed to select two SNPs from each of the top genes implicated for FEV1/FVC or FEV1, nearly all exceeding genome-wide significance. The SNP with the lowest P value in or near each gene was selected. A second SNP, genotyped (instead of imputed) in at least one cohort, was selected with preference for non-synonymous SNPs and SNPs not in strong linkage disequilibrium with the first selected SNP. Only one SNP was available for AGER, PPT2, TSPYL4, and NT5DC1. Four SNPs were selected from two linkage disequilibrium blocks for the largest gene, GPR126. In total, 18 SNPs from nine genes (eight independent loci) implicated for FEV1/FVC and 12 SNPs from seven genes (three independent loci) implicated for FEV1 were tested for replication.
