Chunk #14 — RESULTS — Thioredoxin-interacting protein (TXNIP) is rapidly induced through the UPR

Source

IRE1α induces thioredoxin-interacting protein to activate the NLRP3 inflammasome and promote programmed cell death under irremediable ER stress.

Embedded

yes

Text

Expression of transgenic WT IRE1α (using doxycycline—Dox) causes the protein to spontaneously autophosphorylate as it accumulates (Figure 2E); this leads to complete conversion of cellular XBP1 mRNA to the spliced form (Figure S4C), as occurs under ER stress (Figure S2A)(Han et al., 2009). Activation of WT IRE1α through this maneuver is sufficient to induce TXNIP mRNA (Figure 2D and S4E) and protein (Figure 2F).