Chunk #29 — Methods — Mitochondrial pattern analysis

Source

Ethanol-mediated activation of the NLRP3 inflammasome in iPS cells and iPS cells-derived neural progenitor cells.

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The analysis of mitochondrial distribution was performed by staining fixed cells with 300nM Mitotracker Red CMXRos (Life Technologies) for 20 min, according to the manufacturer’s protocol. Micrographs were taken with a Zeiss LSM700 confocal microscope.