hiPSC lines were generated as described16,17 from skin fibroblasts of subjects with different apoE genotypes or apoE deficiency (Supplementary Table S1). miPSC lines were generated from fibroblasts derived from human apoE3-KI and apoE4-KI mice using a similar protocol16,17. The hiPSCs were maintained under feeder-free condition in mTeSR1 medium or on irradiated SNL feeder cells in hES cell medium consisting of Dulbecco’s modified Eagle’s medium /F12 with 20% Knockout Serum Replacement, 2 mM glutamine, 0.1 mM nonessential amino acids (all from Life Technologies), 4 ng/ml recombinant human basic fibroblast growth factor (bFGF) (PeproTech), and 0.1 mM 2-mercaptoethanol56. hiPSCs were routinely passaged at 1:2–1:4 by brief treatment with Accutase (Millipore) and scraping. The hiPSC protocol was approved by the Committee on Human Research at the University of California, San Francisco.
