the protein-resistant property. We did not alter those patterned substrates to achieve the long-term culture of neurons reported in this work; rather, the stability must reflect either a less oxidative (or otherwise reactive) environment near the cells or a lack of migratory phenotype in these cells. In any event, enabling the culture of human iPSC-derived neurons for more than 100 days, our method enables studies of disease-relevant mechanisms and phenotypes in individual neurons and networks over periods of several months. In particular, we monitored changes in neuronal development, mitochondrial morphology as well as trafficking in living neurons.
