and counting the number of intersecting branches along the circumference of each circle. In a separate experiment, the effect of inhibiting miR-34a on the arbour complexity of differentiating hNPCs transfected with either the anti-miR-34a sequence (GeneCopoeia, HmiR-AN0440-AM03-B) or a control scrambled sequence (GeneCopoeia, CmiR-AN0001-AM03). Since both plasmids co-expressed mCherry as a reporter gene, for these experiments the fixed cells were then immunostained for mCherry to allow complete visualization of transfected cells, and Sholl analysis was done as described above for the miR-34a overexpressing hNPCs.
