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Chunk #0 — METHODS SUMMARY

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Protocadherins mediate dendritic self-avoidance in the mammalian nervous system.
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Transgenic, knock-out and knock-in mouse lines used for this study, as well as methods for marking cells are described in online Methods. Identity of labeled SACs was confirmed by immunolabeling retinas for the cholinergic neuron-specific marker. Dendrite self-crossings were quantified by number of branch overlaps detected in single confocal planes.