Chunk #67 — STAR Methods — Method Details — iMGL Co-culture with Rat Neurons

Source

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.

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Text

Rat hippocampal or cortical neurons were cultured for 21 days with 50% media change every 3–4 days. iMGLs were cultured with neurons at a 1:5 ratio (1 × 106 iMGL to 5 × 106 neurons) in 50% iMGL and 50% NB medium. After 3 days, iMGLs were collected for RNA isolation.