iPSC lines derived from different donors (Figure 3B). qPCR analyses on selected genes (MEG3, WNT3A, SOX17, and SNAI2) replicated these results (Figure 3C). GO analysis of these 167 differentially expressed genes primarily indicated biological processes related to early development, which was confirmed using a PANTHER over-representation test (Figures 3D and 3D′). This suggests that donor-based variability has a major influence on the expression of genes related to pluripotency and lineage priming.
