Chunk #14 — Material and Methods — Operant self-Administration procedure for 20% or 10% ethanol

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Varenicline, a partial agonist at neuronal nicotinic acetylcholine receptors, reduces nicotine-induced increases in 20% ethanol operant self-administration in Sprague-Dawley rats.

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as a measure of nonspecific behavior. Rats continued on the FR3 protocol with 20% ethanol as the reinforcer for a minimum of 24 sessions. Any animals that failed to receive any reinforcers (<1) during the last 5 operant sessions were excluded from further study. When the animals had maintained a stable baseline (20 sessions) blood samples were collected from the lateral tail vein immediately following a 30-minute FR3 session, samples were then prepared and analyzed as described above.