Chunk #1 — Methods Summary — Lentiviral Infections

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Induction of human neuronal cells by defined transcription factors.

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Lentiviral production and fibroblast infections were performed as described previously13. Primary fibroblasts and pluripotent stem cells were infected with concentrated lentivirus and treated with doxycycline (dox, 2 µg/µL) 16–24 hours later.