The MS data were collected in the ‘Triple-Play” mode (MS scan, Zoom scan, and MS/MS scan). Each sample was analyzed with this approach; samples were injected only once. The acquired data were then filtered and analyzed by licensed software developed and described by Higgs et al. (2005). Searches against the IPI (International Protein Index) and the Non-Redundant (NCBI) databases were carried out using both the SEQUEST and X!Tandem algorithms.
