Whereas KCTD13 gRNA 2 and 3 were efficacious in dCas9−VP64 NPC lines from two individuals (Figure 2A), no gRNAs produced significant effects in dCas9−KRAB NPCs (using the stringent multiple comparison statistical analysis used throughout; antibiotic selection for dCas9−KRAB) (Figure 3A). Consistent with null effects observed in dCas9−VP64 NPCs (Figure 2B), none of the TOAK2 gRNAs modulated transcription in dCas9−KRAB NPCs (antibiotic selection for dCas9−KRAB) (Figure 3B).
