KCNJ3, encoding GIRK1, which are required to form heterotetrameric, functional channels [14, 38]. Detection of mRNA in individual cells by scRNAseq underestimates expression, so the cluster identified as neurons likely expresses markers more uniformly than observed here. We conclude that this cluster of cells expresses components required for physiological activity, and that other cells, expressing subsets of these neuronal markers, are alternate products of induction, which we label as “transitional” neurons (Fig. 1C), since reprogramming is not identical to differentiation [39, 40]. Therefore, we focused gene expression analysis on the phenotypically neuronal subset of cells.
