with self-identification as the numbers of markers decrease, for example, the cluster membership that corresponds best to self identified Amerindian ancestry (pop 4) decreased from 0.94 (128 In4) to 0.88 (24 In4) (Table 2). However, the difference is more pronounced for the estimated contribution from pop5 (corresponding to South Asian background) in the South Asian population (0.75/0.68/0.70/0.59/0.55). The increased uncertainty for South Asian contribution may be explained by the relatively low Fst values between South Asian and European/East Asian populations observed for the In4 markers (Table 1) that in turn reflects the selection criteria (see Methods).
