For quantitation in human tissue, immunostained sections were analyzed using the computer image analysis system MicroComputer Imaging Device (MCID; Imaging Research, St. Catherines, Ontario, Canada) and blinded assessment of optical density measurements were obtained relative to the proportional area for AT180 and NeuN immunostaining in frontal cortex grey matter (three separate readings per case) as previously described [19]. For quantitation in mouse brain tissue, the number of neurons with cytoplasmic pSRRM2 inclusions were manually counted across the entire section (two sections per animal). Data were averaged and are represented as means ± SEM. A two-tailed Student’s t test was used to assess differences in staining intensity between experimental groups. Statistical analysis and graphing was performed using the Prism V8.3 software package (GraphPad).
