Single subject contrasts were used for whole brain 2×2 ANOVAs in SPM with valence (reward/loss) and group (FH−/FH+) as factors for each NAcc. To identify brain regions showing significant group differences in functional coupling, two contrasts were created: FH+ minus FH−, and FH− minus FH+. Statistical significance was established at p<0.05, corrected for multiple comparisons at the cluster level. For identified ROIs, connectivity was extracted from individual PPI maps.
