Chunk #15 — Materials and Methods — Data analysis — Spike phase estimation

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Low and High Gamma Oscillations in Rat Ventral Striatum have Distinct Relationships to Behavior, Reward, and Spiking Activity on a Learned Spatial Decision Task.

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To obtain the mean phase angle of putative FSI spiking relative to ongoing gamma oscillations, LFPs were first band-pass filtered (fourth order Chebyshev, r = 0.5, MATLAB filter and filtfilt routines; 45–55 Hz for gamma-50, 70–85 Hz for gamma-80) before a Hilbert transform was applied to obtain the instantaneous phase angle. A histogram of spike counts in each of 10° phase bins was constructed, and Rayleigh's r test used to determine the significance of deviations from the uniform distribution (Fisher, 1993).