Analyses were conducted in Excel (2003, Microsoft) or Graphpad Prism (Graphpad Software; La Jolla, CA) and are reported as mean ± standard error of the mean (SEM). BECs (mg/dl) and ethanol dose (g/kg) were analyzed by ANOVA. All two group comparisons, specifically cell counts and immunoreactivity, were analyzed by t-test. Behavioral intoxication scores were analyzed by Kruskall-Wallis. In all analyses, differences were considered significant at p<0.05.