Saccharin intake ranged between 3.5 and 4.8mL/day with preference ratios between 77% and 81% between genotypes (Fig. 4B). However, when drinking data were plotted in weekly averages (Fig. 4A and B), there was a significant interaction of genotype and time (F(10,100) = 2.299). Post-hoc tests revealed that this interaction was driven by a significant difference in alcohol intake between the WT and 3xTg mice during the first week of self-administration, with 3x-Tg mice consuming more alcohol than their wild-type counterparts. This difference was not significantly different by week 2 and by week 7, both groups consumed similar amounts of alcohol. No differences were observed in body weight between genotypes or treatment groups (Fig. 4C). Next, during a 3-week alcohol-free period, 7-month-old mice were tested on a battery of behavioral tests in randomized order.
