Assuming that the CVs reflect measurement error, the results indicated that the sequential method (temporally forward) yielded lower error than the independent method in longitudinal data analysis and thus was used in the remaining analyses to characterize developmental change in regional brain volume over the rescan interval. ROIs exhibiting significant volume changes from MRI 1 to MRI 2 are presented as “spaghetti” plots (Figures 5–7 and Table 2).