In brief, hepatic homogenates were incubated with 0.2 ml of TCA solution [15% (wt/vol)]-thiobarbituric acid (TBA) [0.375% (wt/vol)]-hydrogen chloride (HCl) (0.25 N) in a boiling water bath for 10 min. After centrifugation at 1,000 rpm for 5 min, the resulting supernatant was used to determine the formation of TBARS by evaluating absorbance at 535 nm. Malondialdehyde (MDA) was treated as above and served as a standard (19).
