Human fibroblast cells were reprogrammed by the UCHC Stem Cell Core using retrovirus constructs expressing 5 reprogramming factors (OCT4, SOX2, KLF4 c-MYC, and LIN28). iPS cell colonies were identified based on characteristic morphology approximately 2–4 weeks after viral transduction. Selected colonies were expanded as separate clones and the expression of pluripotency markers SSEA-3/4 and NANOG was verified by the UCHC Stem Cell Core using immunocytochemistry.
