The use of bisulfite modification coupled with deep genome sequencing appears to be a step forward in assessing effects of ethanol on the methylome. While the article did not provide much detail on how sequencing data were analyzed, at least the DMRs identified by these methods were validated by reversal after 5-azaC treatment to block methylation. By combining methylation analysis with gene expression studies, the authors were able to apply pathway analysis and transcription factor binding site enrichment to determine patterns of response to ethanol.
