other factors like protein expression and alternative splicing. As a result, we leveraged SNP weights, where available, for protein expression and splicing to also perform a proteome-wide association study (PWAS) and an alternative splicing based test (spliceWAS). Notably, PWAS and spliceWAS analyses have not previously be published for AN. Genes were prioritised based on evidence from mRNA, protein, and alternative isoform expression and then subjected to further in silico analyses related to overrepresentation in biological pathways. The AN GWAS utilised in this study was a meta-analysis encompassing European ancestry cohorts that totalled 16 992 cases and 55 525 controls. In the AN GWAS, case status was mostly ascertained from online questionnaires or structured interviews based on standardised clinical criteria, for example, DSM-IV, whilst the UK Biobank derived cases were self-reported. Further details related to collection of samples, phenotype acquisition, and GWAS approach are described in the original publication (Watson et al., 2019).
