FN-439 (EMD Biosciences, Gibbstown, NJ; Prod#444250) was soluble in aCSF (pH of 7.4 and compressed of 145 mM NaCl, 2.8 mM KCl, 1.2 mM MgCl2,1.2 mM CaCl2, 5.4 mM d-Glucose and 0.25 mM ascorbic acid; Alvarez-Jaimes, Stouffer and Parsons, 2009; Nealey, Smith, Davis, Smith and Walker, 2011). In Condition 2, FN-439 or aCSF were infused at a rate of 0.15 μl/hr, whereas the animals in Condition 3 received FN-439 or aCSF via internal cannulae (31 gauge that extended 1 mm past the guide cannula) at a volume and rate of 1 μl/side over 60 seconds.
