The assay was used to examine the sequence specificity and DNA-binding affinity of representative members from each of the four remaining ARID subfamilies (Figure 4). ARID2 is the only member of its subfamily. A full-length human cDNA has not been reported thus far, but Genbank sequences predict an ARID consensus sequence at the N-terminus of the ARID2 gene product. Isolation of N-terminal cDNA sequence by RT–PCR from the human liver cell line HepG2 confirms the presence of the ARID in the transcript (accession number AY727870.1). Studies on mammalian ARID2 have not yet been reported, but the protein is an apparent ortholog of the Drosophila ARID protein BCDNA:GH12174 (CG3274). Both proteins contain an RFX domain, which is an additional DNA-binding domain [reviewed in (21)]. Interestingly, the protein product of Drosophila BCDNA:GH12174 was recently found to be a component of the SWI/SNF-like complex PBAP, and was designated BAP170 (22). This complex is distinguished from the BAP SWI/SNF-like complex in part by its lack of Osa. This finding extends the role of ARID-containing subunits as components of SWI/SNF-related chromatin-remodeling complexes. Analysis of ARID2
