Since the publication of DrugBank 5.0, there have been 3446 new drug–target entries added, with 546 distinct new bioentities (proteins, genes, other cellular components) identified. This has led to a total of 4939 distinct bioentities representing drug targets, which is an 8% increase compared to the 4563 in DrugBank 5.0. For new protein entities, we use UniProt keywords associated with each UniProt identifier to annotate the protein targets. Roughly 33% of these are kinases, suggesting that kinases have represented a particularly valuable target class in the last six years. Certain cellular functions were also highly represented, with nucleotide binding (44%) and transferase (40%) activities being common among newly added target entities. In addition to drug targets, DrugBank 6.0 has added 2550 new drug-enzyme entries, 1560 new drug-transporter entries and 550 new drug-carrier entries. These include 65, 60 and 14 new distinct bioentities, respectively. The total number of distinct enzymes, transporters and carriers has increased to 836.
