Chunk #15 — Introduction — Rationale for the present study: Mechanisms of Alzheimer’s disease identified as upstream regulators of alcohol-sensitive protein networks in C57BL/6J mice
al., 2017; Schroeder, Olive, Koenig, & Hodge, 2003; Schroeder et al., 2008; Ueno et al., 2001). In each study, nondependent mice consumed alcohol (10%, v/v) vs water or water only in the home-cage for ~1 month (Fig. 1A). Changes in the AMY or PFC neuroproteome were assessed 24-h post alcohol via two-dimensional differential in-gel electrophoresis (2D-DIGE) with protein identification by MALDI/TOF/TOF mass spectrometry (Fig. 1B). Alcohol intake (~12g/kg/day) consistently (n = 4 replicate gels per brain region) altered an array of fundamental protein networks that regulate a range of diseases, biological functions, molecular and cellular functions, and developmental processes suggesting that alcohol may impact health and vulnerability to disease across the lifespan (Agoglia & Hodge, 2017; Salling et al., 2016).
