Chunk #57 — STAR Methods — Quantification and Statistical Analysis — Comparison of primary cortical cell and in vitro interneuron populations

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Single-Cell Profiling of an In Vitro Model of Human Interneuron Development Reveals Temporal Dynamics of Cell Type Production and Maturation.

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Seven populations of primary cortical cells were sorted based on SOX2 and PAX6 antibody staining, with n≥3 unique specimens and n = 9–16 technical replicates per population. Six populations of 100 in vitro cells were collected based on Cit fluorescence and stage (D26, D54, and D100), with N = 3–11 replicates per population. In vivo and in vitro cells were compared as described below.