In this study, we aimed to elucidate the relationship between ethanol treatment and the expression of Cd14 in the hippocampus as well as to identify interacting genes and pathways through which Cd14 regulates ethanol responses. We found that Cd14 shows variable expression in the hippocampus among the BXDs and is cis-regulated in the Affymetrix hippocampus dataset, which makes this gene an excellent candidate for study as a modifier that regulates expression of other transcripts and biologic phenotypes [34]. Cd14 was not found to be cis-regulated in the Illumina hippocampus datasets that were used for this paper, but it was found to be cis-regulated in the Illumina hippocampus combined dataset that has more samples per strain and increased power, allowing the cis-eQTL to be detected (S1A Fig). To verify that Cd14 is cis-regulated, we also checked all available BXD datasets in GeneNetwork and found that Cd14 is also a cis-regulated in the neocortex, mid-brain, prefrontal cortex, striatum, ventral tegmental area, bone, kidney, progenitor cells, and spleen among BXD mice (S1 Fig), which were generated using different microarray platforms. This data strongly
