Rotarod. For each trial, each mouse was placed on its own segment of the rotarod facing the back wall. The rod was spinning at five revolutions per minute (r.p.m.) at the beginning of the test and accelerated to 25 r.p.m. The mouse remained on the rotarod until it fell off. Both the length of time and the speed of the rotarod when the mouse falls were recorded. On Day 1 (training day), each mouse was given 10 trials on the rotarod and data were recorded. Because the behavior of the mice reached a plateau after five trials, the last three were used to calculate the mean for the training day for both speed and time on rotarod in seconds. On Days 2 and 3, only three trials were given and all were used to compute the mean for each condition (saline vs. ethanol). All mean times were recorded. In addition, several computed measures were also determined: training mean–saline mean (to determine the effects of the injection, repeated exposure and prior tests on the rotarod score), training minus ethanol (to determine
