Chunk #24 — MATERIALS AND METHODS — Light microscopic immunocytochemistry

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Endogenous GluR1-containing AMPA receptors translocate to asymmetric synapses in the lateral amygdala during the early phase of fear memory formation: an electron microscopic immunocytochemical study.

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In order to confirm that immunoreactivity of the amygdala to the GluR1 and GluR2/3 subunits was as observed previously (Farb et al., 1995), Vibratome sections of naïve animals were used to immunolabel the tissue by the avidin-biotin complex, by using Vector’s Elite kit for rabbit IgGs (Vector, Burlingame, CA, cat. no. PK-6200). Substrate for peroxidase was 0.3% 3,3′-diaminobenzidine HCl with 0.03% H2O2, and the buffer was 0.01 M phosphate buffer (pH 7.4) containing 0.9% NaCl.