We note that 13 of the 15 detected point mutations in the tumor suppressor NF1 occurred in the 93 ovarian samples that had not undergone genome doubling (P = 0.002; Fisher's exact test), and these mutations were uniformly homozygous (not shown). This is consistent with selection for recessive inactivation of NF1, a typical pattern for a tumor suppressor gene. It also suggests that non-genome-doubled ovarian carcinoma samples evolved via a distinct trajectory, rather than being precursors to doubled samples. If not, many NF1 mutations would be homozygous with multiplicity > 1 in doubled samples, as is seen for TP53.
