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Chunk #12 — Materials and Methods — iN cell derivation from iPSCs

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μNeurocircuitry: Establishing models of neurocircuits with human neurons.
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Preparation of iPSC from human primary lymphocytes using Sendai viral vectors (CytoTune™, Life Technologies, Grand Island, NY) has been described27,28. All source biomaterials were de-identified repository specimens and therefore are exempt from human subjects regulations. Feeder-free mTeSR™1 medium (Stem Cell Technologies) was used.