be important for future works to use this model system to examine other AD related phenotypes, such as neuroinflammation, gliosis, DNA damage, U1 tangles [88] and synaptic dysfunction. Also, without a means of tissue perfusion, the organoid suffers from the same issues as primary slice culture, in that the distance from the culture medium interface is correlated with tissue necrosis. There is currently great interest in the combination of three-dimensional neural culture systems with artificial blood-brain barrier technology [89–91], to address this issue.
