Two of the genes implicated in this work have been previously implicated in memory and or ethanol-related behaviors. shi, the fly homolog of Dynamin (van der Bliek and Meyerowitz 1991), plays a role in endocytosis, which is critical for synaptic transmission in MB neurons during memory formation (McGuire et al. 2001; Kasuya et al. 2009) and in ethanol tolerance (Krishnan et al. 2012). We have also previously shown that expression of a temperature-sensitive form of shi in MB neurons disrupts ethanol cue memory at higher temperatures (Kaun et al. 2011). Stat92E, a transcription factor in the JAK/STAT signaling pathway, plays a role in long-term memory in Drosophila (Copf et al. 2011), and we have recently shown that reducing Stat92E in MB neurons similarly reduces ethanol cue memory (Petruccelli et al. 2018). Other targets identified in this work have been implicated in long-term memory in Drosophila (Figure S11). Similarly, a recent study showing the effects of acute response to alcohol shows some overlap in transcriptomic targets (Figure S11) (Signor and Nuzhdin 2018).
