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Chunk #42 — MATERIALS AND METHODS — Genotyping

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Nicotinic acetylcholine receptor beta2 subunit gene implicated in a systems-based candidate gene study of smoking cessation.
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Among the 1528 total SNPs, we excluded 41 SNPs with a call rate of zero and 57 SNPs with an observed MAF <0.01. There were a total of 1430 SNPs available for analysis (1198 SNPs within the candidate genes and 232 AIMs). Of the remaining SNPs, 97% had a SNP call rate ≥95%. Of the 1198 SNPs within the candidate genes, a total of eight SNPs had a P-value of <0.0001 from an exact test of Hardy–Weinberg proportions. The genotyping calls for these SNPs were re-examined, confirmed, and included in the analysis (76–79).