The Affymetrix Axiom Biobank Genotyping Array (Affymetrix, Inc.) was used for rare and common variant genotyping and analysis, capturing variants with a minor allele frequency (MAF) greater than 0.005%. Assays and genotype calls were made according to protocols provided by Affymetrix (Affymetrix, Inc.). A number of standard genotyping quality control steps were conducted (Anderson et al., 2010) using PLINK 1.07 (Purcell et al., 2007) in order to assess genotyping sample quality and accuracy, as well as sample identities for the original dataset of 428 individuals and 628,679 single nucleotide variants (SNVs). Degree of relatedness estimations identified two first-degree siblings. Given that analyses of alcohol challenge data would be restricted to individuals in the alcohol beverage condition, the sibling who had been assigned to the active placebo condition was removed in order to ensure that the sample was composed of unrelated individuals. Gender checks of sample identities were evaluated, and 7 individuals with unresolved discrepant sex codes were excluded.
