Gene open reading frames (ORFs) were cloned into the AccIII‐XbaI or NotI-ClaI restriction endonuclease sites of pcDNA3‐V1/V2 to produce either amino- or carboxyl-terminal split‐Venus‐tagged proteins, respectively. The ZBTB16 constructs were established previously24. HEK-293 cells were seeded into a 24‐well culture plate and transfected with 50 ng/well of each construct using lipofectamine 2000 (Thermo Fisher Scientific). Total Venus fluorescence was captured 30 hours after transfection of cells using a Zeiss MBQ 52 AC burner with a 4x lens on an Axiovert 40 CFL Trinocular Inverted fluorescence microscope and a ProgRes camera and software (Jenoptik). The level of fluorescence was normalised to a nonfluorescent control constituted by coexpressing split-tagged ZBTB16 and the Eukaryotic initiation factor 2α and/or SUMO1 tagged with each half of the split fluorophore. The level of fluorescence was averaged from three images per experiment. The data was analysed using the ImageJ software (NIH and LOCI). Micrographs were captured from cells seeded and transfected on sterile coverslips (Menzel‐Glaser, 12 mm diameter, #1.5, Thermo Fisher Scientific) within a 24-well culture plate. After 30 h cell nuclei were stained with Hoechst (Invitrogen),
